Morphological and Mechanical Properties of Osteosarcoma Microenvironment Cells Explored by Atomic Force Microscopy
نویسندگان
چکیده
منابع مشابه
Study of the mechanical properties of single cells as biocomposites by atomic force microscopy
Atomic force microscopy (AFM) is a unique method to study a single cell surface layer as a biocomposite. This chapter focuses on the advantages of two AFM modes, namely force spectroscopy (providing the information about local elastic properties of cells) and lateral force microscopy (LFM, revealing mechanical heterogeneity of cells surface), to make quantitative analysis of single cells mechan...
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Atomic force microscopy (AFM) allows the acquisition of high-resolution images and the measurement of mechanical properties of living cells under physiological conditions. AFM cantilevers with blunted pyramidal tips are commonly used to obtain images of living cells. Measurement of mechanical properties with these tips requires a contact model that takes into account their blunted geometry. The...
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Atomic force microscopy (AFM) is a modern imaging technique that generates height representations of a sample surface with subnanometer resolution. Since 1986 it has evolved into a versatile tool which also provides maps of elastic and viscoelastic properties. AFM offers the opportunity to image samples with or without treatment in various environments. Aim of this work is a short description o...
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The organization of filamentous actin and myosin II molecular motor contractility is known to modify the mechanical properties of the cell cortical actomyosin cytoskeleton. Here we describe a novel method, to our knowledge, for using force spectroscopy approach curves with tipless cantilevers to determine the actomyosin cortical tension, elastic modulus, and intracellular pressure of nonadheren...
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Active cell migration and invasion is a peculiar feature of glioma that makes this tumor able to rapidly infiltrate into the surrounding brain tissue. In our recent work, we identified a novel class of glioma-associated-stem cells (defined as GASC for high-grade glioma--HG--and Gasc for low-grade glioma--LG) that, although not tumorigenic, act supporting the biological aggressiveness of glioma-...
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ژورنال
عنوان ژورنال: Analytical Sciences
سال: 2016
ISSN: 0910-6340,1348-2246
DOI: 10.2116/analsci.32.1177